One end of Domain Road will be closed for five years due to construction of the Melbourne Metro Rail tunnel and Domain station.
As of today the South Yarra thoroughfare is already closed between St Kilda Road and the western end of Edmund Herring Oval.
After roadworks to install new rails were completed during the school holidays, Route 58 trams now travel along St Kilda Road and Toorak Road instead of Domain Road and Park Street.
New platform stops will be built on St Kilda Road at Toorak Road, and on Toorak Road near Millswyn Street, Yarra Trams says.
Domain Road joins a number of other streets facing up to five years closure due to the metro project.